Para acceder a los documentos con el texto completo, por favor, siga el siguiente enlace: http://hdl.handle.net/2117/20906

The effect of the composition of PLA films and lactate release on glial and neuronal maturation and the maintenance of the neuronal progenitor niche.
Álvarez, Zaída; Mateos Timoneda, Miguel Ángel; Hyroššová, Petra Hyroššová; Castaño Linares, Óscar; Planell Estany, Josep Anton; Perales, José C.; Engel López, Elisabeth; Alcántara, Soledad
Universitat Politècnica de Catalunya. Departament de Ciència dels Materials i Enginyeria Metal·lúrgica; Institut de Bioenginyeria de Catalunya; Universitat Politècnica de Catalunya. BIBITE - Biomaterials, Biomecànica i Enginyeria de Teixits
To develop tissue engineering strategies useful for repairing damage in the central nervous system (CNS) it is essential to design scaffolds that emulate the NSC niche and its tight control of neural cell genesis, growth, and differentiation. In this study we tested two types of poly l/dl lactic acid (PLA95/5 and PLA70/30), a biodegradable material permissive for neural cell adhesion and growth, as materials for nerve regeneration. Both PLA were slightly hydrophobic and negatively charged but differed in crystallinity, stiffness and degradation rate. PLA95/5 films were highly crystalline, stiff (GPa), and did not degrade significantly in the one-month period analyzed in culture. In contrast, PLA70/30 films were more amorphous, softer (MPa) and degraded faster, releasing significant amounts of lactate into the culture medium. PLA70/30 performs better than PLA95/5 for primary cortical neural cell adhesion, proliferation and differentiation, maintaining the pools of neuronal and glial progenitor cells in vitro. l-lactate in the medium recapitulated PLA70/30's maintenance of neuronal restricted progenitors but did not sustain bipotential or glial restricted progenitors in the cultures, as occurred when neural cells were grown on PLA70/30. Our results suggest that PLA70/30 may mimic some of the physical and biochemical characteristics of the NSC niche. Its mechanical and surface properties may act synergistically in the modulation of bipotential and glial restricted progenitor phenotypes, while it is l-lactate, either added to the medium or released by the film that drives the maintenance of neuronal restricted progenitor cell phenotypes.
Peer Reviewed
Àrees temàtiques de la UPC::Enginyeria dels materials
Materials--Biodegradation
Biomedical materials
Polylactic acid
Degradation
Neurons
Progenitors
Lactate
Glial cells
NSC niche
Biodegradació -- Aplicacions en medicina
Enginyeria de teixits
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/publishedVersion
Artículo
         

Mostrar el registro completo del ítem

Documentos relacionados

Otros documentos del mismo autor/a

Álvarez Pinto, Zaída; Castaño Linares, Óscar; Castells, Alba; Mateos Timoneda, Miguel Ángel; Planell Estany, Josep Anton; Engel López, Elisabeth; Alcántara, Soledad
Sachot, Nadège; Castaño Linares, Óscar; Mateos Timoneda, Miguel Ángel; Engel López, Elisabeth; Planell Estany, Josep Anton
Mateos Timoneda, Miguel Ángel; Castaño Linares, Óscar; Planell Estany, Josep Anton; Engel López, Elisabeth
Sachot, Nadège; Mateos Timoneda, Miguel Ángel; Planell Estany, Josep Anton; Velders, A. H.; lewandowska, M.; Engel López, Elisabeth; Castaño Linares, Óscar
Mattotti, Marta; Alvarez, Zaida; Ortega, Juan A.; Planell Estany, Josep Anton; Engel López, Elisabeth; Alcántara, Soledad