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Nanocharacterization of Soft Biological Samples in Shear Mode with Quartz Tuning Fork Probes
Otero Díaz, Jorge; González Claramonte, Laura; Puig i Vidal, Manuel
Universitat de Barcelona
Quartz tuning forks are extremely good resonators and their use is growing in scanning probe microscopy. Nevertheless, only a few studies on soft biological samples have been reported using these probes. In this work, we present the methodology to develop and use these nanosensors to properly work with biological samples. The working principles, fabrication and experimental setup are presented. The results in the nanocharacterization of different samples in different ambients are presented by using different working modes: amplitude modulation with and without the use of a Phase-Locked Loop (PLL) and frequency modulation. Pseudomonas aeruginosa bacteria are imaged in nitrogen using amplitude modulation. Microcontact printed antibodies are imaged in buffer using amplitude modulation with a PLL. Finally, metastatic cells are imaged in air using frequency modulation.
Microscòpia de força atòmica
Citologia
Nanotecnologia
Biomolècules
Bacteris
Bioenginyeria
Atomic force microscopy
Cytology
Nanotechnology
Biomolecules
Bacteria
Bioengineering
cc-by (c) Otero Díaz, Jorge et al., 2012
http://creativecommons.org/licenses/by/3.0/es
Artículo
info:eu-repo/semantics/publishedVersion
MDPI Publishing
         

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