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Ion-selective electrodes to monitor osteoblast-like cellular influence on the extracellular concentration of calcium
Gustavsson, Johan; Planell Estany, Josep Anton; Engel López, Elisabeth
Universitat Politècnica de Catalunya. Departament de Ciència dels Materials i Enginyeria Metal·lúrgica; Universitat Politècnica de Catalunya. BIBITE - Biomaterials, Biomecànica i Enginyeria de Teixits
In bone tissue engineering, the composition of the ionic extracellular environment (IEE) can determine both cellular fate and a biomaterial ’ s development and performance. Therefore, precise control of the IEE and a perfect understanding of the dynamic changes that it can be subject to due to cellular activity ishighlydesired.Toachievethis,weinitiallymonito redhowtwostandardosteoblast-likecellmodelsthat expressedeitherhighorlowalkalinephosphataseactivity-SAOS-2andMG63cells,respectively-affected the extracellular concentrations of calcium and phosphate during long-term cultures. It was observed that cellular in fl uence onthe IEE varied greatly between the two models and could be linked to the capac- ity of cells to deposit calcium in the extracellular matri x. Miniaturized ion-selective electrodes that could allow for real-time monitoring ofcalcium in aminimally invasive waywere then constructed.The electro- des were characterized in standard in vitro cell culture environments, prior to being successfully applied for periods of 24h, to record the dynamics of cell-induced deposition of calcium in the extracellular matrix, while using osteogenic media of either high or low concentrations of phosphate. As a result, this study provides the background and technological means for the non-destructive evaluation of the IEE in vitro and allows for the optimization and development of better models of bone tissue construction
Peer Reviewed
Àrees temàtiques de la UPC::Enginyeria dels materials
Tissue engineering
Osteoblasts
Extracellular ions
Ion-selective electrode
MG63
Mineralization
Osteoblasts
Saos-2
Sensor
Tissue engineering
Ossos -- Regeneració
Enginyeria de teixits
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/publishedVersion
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