Para acceder a los documentos con el texto completo, por favor, siga el siguiente enlace: http://hdl.handle.net/2445/44837

Glibenclamide enhances neurogenesis and improves long-term functional recovery after transient focal cerebral ischemia
Ortega González, Fco. Javier; Jolkkonen, J.; Mahy Gehenne, Josette Nicole; Rodríguez Allué, Manuel José
Universitat de Barcelona
Glibenclamide is neuroprotective against cerebral ischemia in rats. We studied whether glibenclamide enhances long-term brain repair and improves behavioral recovery after stroke. Adult male Wistar rats were subjected to transient middle cerebral artery occlusion (MCAO) for 90 minutes. A low dose of glibenclamide (total 0.6mg) was administered intravenously 6, 12, and 24 hours after reperfusion. We assessed behavioral outcome during a 30-day follow-up and animals were perfused for histological evaluation. In vitro specific binding of glibenclamide to microglia increased after pro-inflammatory stimuli. In vivo glibenclamide was associated with increased migration of doublecortin-positive cells in the striatum toward the ischemic lesion 72 hours after MCAO, and reactive microglia expressed sulfonylurea receptor 1 (SUR1) and Kir6.2 in the medial striatum. One month after MCAO, glibenclamide was also associated with increased number of NeuN-positive and 5-bromo-2-deoxyuridine-positive neurons in the cortex and hippocampus, and enhanced angiogenesis in the hippocampus. Consequently, glibenclamide-treated MCAO rats showed improved performance in the limb-placing test on postoperative days 22 to 29, and in the cylinder and water-maze test on postoperative day 29. Therefore, acute blockade of SUR1 by glibenclamide enhanced long-term brain repair in MCAO rats, which was associated with improved behavioral outcome.
Isquèmia cerebral
Micròglia
Regeneració del sistema nerviós
Cerebral ischemia
Microglia
Nervous system regeneration
(c) Ortega González, Fco. Javier et al., 2013
Artículo
info:eu-repo/semantics/acceptedVersion
International Society for Cerebral Blood Flow and Metabolism
         

Mostrar el registro completo del ítem

Documentos relacionados

Otros documentos del mismo autor/a

Ortega González, Fco. Javier; Jolkkonen, J.; Mahy Gehenne, Josette Nicole; Rodríguez Allué, Manuel José
Ortega González, Fco. Javier; Vidal Taboada, José Manuel; Mahy Gehenne, Josette Nicole; Rodríguez Allué, Manuel José
Rodríguez Allué, Manuel José; Martinez-Moreno, M.; Ortega González, Fco. Javier; Mahy Gehenne, Josette Nicole
Ortega González, Fco. Javier; Gimeno-Bayon, Javier; Espinosa-Parrilla, J.F.; Carrasco Jordan, Josep Lluís; Batlle, M.; Pugliese, Marco; Mahy Gehenne, Josette Nicole; Rodríguez Allué, Manuel José
Rodríguez Allué, Manuel José; Martinez-Moreno, M.; Ortega González, Fco. Javier; Mahy Gehenne, Josette Nicole
 

Coordinación

 

Patrocinio