dc.contributor |
Universitat de Barcelona |
dc.contributor.author |
Parvas, Maryam |
dc.contributor.author |
Bueno i Torrens, David, 1965- |
dc.date |
2013-05-27T17:43:04Z |
dc.date |
2013-05-27T17:43:04Z |
dc.date |
2010 |
dc.date |
2013-05-27T17:43:04Z |
dc.identifier.citation |
2156-8456 |
dc.identifier.citation |
588554 |
dc.identifier.uri |
http://hdl.handle.net/2445/43778 |
dc.format |
7 p. |
dc.format |
application/pdf |
dc.language.iso |
eng |
dc.publisher |
Scientific Research Publishing |
dc.relation |
Reproducció del document publicat a: http://dx.doi.org/10.4236/abb.2010.14041 |
dc.relation |
Advances in Bioscience and Biotechnology , 2010, vol. 1, p. 315-321 |
dc.relation |
http://dx.doi.org/10.4236/abb.2010.14041 |
dc.rights |
cc-by (c) Parvas, Maryam et al., 2010 |
dc.rights |
info:eu-repo/semantics/openAccess |
dc.rights |
http://creativecommons.org/licenses/by/3.0/es |
dc.subject |
Cervell |
dc.subject |
Sistema nerviós central |
dc.subject |
Neurobiologia del desenvolupament |
dc.subject |
Brain |
dc.subject |
Central nervous system |
dc.subject |
Developmental neurobiology |
dc.title |
The embryonic blood-CSF barrier has molecular elements for specific glucose transport and for the general transport of molecules via transcellular routes. |
dc.type |
info:eu-repo/semantics/article |
dc.type |
info:eu-repo/semantics/publishedVersion |
dc.description.abstract |
In vertebrates, early brain development takes place at the expanded anterior end of the neural tube, which is filled with embryonic cerebrospinal fluid (E-CSF). We have recently identified a transient blood-CSF barrier that forms between embryonic days E3 and E4 in chick embryos and that is responsible for the transport of proteins and control of E-CSF homeostasis, including osmolarity. Here we examined the presence of glucose transporter GLUT-1 as well the presence of caveolae-structural protein Caveolin1 (CAV-1) in the embryonic blood-CSF barrier which may be involved in the transport of glucose and of proteins, water and ions respectively across the neuroectoderm. In this paper we demonstrate the presence of GLUT-1 and CAV-1 in endothelial cells of blood vessels as well as in adjacent neuroectodermal cells, located in the embryonic blood-CSF barrier. In blood vessels, these proteins were detected as early as E4 in chick embryos and E12.7 in rat embryos, i.e. the point at which the embryonic blood-CSF barrier acquires this function. In the neuroectoderm of the embryonic blood-CSF barrier, GLUT-1 was also detected at E4 and E12.7 respectively, and CAV-1 was detected shortly thereafter in both experimental models. These experiments contribute to delineating the extent to which the blood-CSF embryonic barrier controls E-CSF composition and homeostasis during early stages of brain development in avians and mammals. Our results suggest the regulation of glucose transport to the E-CSF by means of GLUT-1 and also suggest a mechanism by which proteins are transported via transcellular routes across the neuroectoderm, thus reinforcing the crucial role of E-CSF in brain development. |