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Biphasic calcium sulfate dihydrate/iron-modified alpha-tricalcium phosphate bone cement for spinal applications : in vitro study
Vlad, María Daniela; Valle Mendoza, Luis Javier del; Poeata, I.; López López, José; Torres Cámara, Ricardo; Barraco Serra, Marc; Fernández Aguado, Enrique
Universitat Politècnica de Catalunya. Departament d'Enginyeria Química; Universitat Politècnica de Catalunya. Departament de Física i Enginyeria Nuclear; Universitat Politècnica de Catalunya. Departament de Mecànica de Fluids; Universitat Politècnica de Catalunya. Departament de Ciència dels Materials i Enginyeria Metal·lúrgica; Universitat Politècnica de Catalunya. GRICCA - Grup Interdepartamental per a la Col.laboració Científica Aplicada
Premi del "National University Research Council" del Ministeri d'Educació i Recerca de Romania, a autors romanesos per l'impacte de la seva recerca.
In this study, the cytocompatibility of new ‘iron-modified/alpha-tricalcium phosphate (IM/α-TCP) and calcium sulfate dihydrate (CSD)’ bone cement (IM/α-TCP/CSD-BC) intended for spinal applications has been approached. The objective was to investigate by direct-contact osteoblast-like cell cultures (from 1 to 14 days) the in vitro cell adhesion, proliferation, morphology and cytoskeleton organization of MG-63 cells seeded onto the new cements. The results were as follows: (a) quantitative MTT-assay and scanning electron microscopy (SEM) showed that cell adhesion, proliferation and viability were not affected with time by the presence of iron in the cements; (b) double immunofluorescent labeling of F-actin and α-tubulin showed a dynamic interaction between the cell and its porous substrates sustaining the locomotion phenomenon on the cements’ surface, which favored the colonization, and confirming the biocompatibility of the experimental cements; (c) SEM-cell morphology and cytoskeleton observations also evidenced that MG-63 cells were able to adhere, to spread and to attain normal morphology on the new IM/α-TCP/CSD-BC which offered favorable substratum properties for osteoblast-like cells proliferation and differentiation in vitro. The results showed that these new iron-modified cement-like biomaterials have cytocompatible features of interest not only as possible spinal cancellous bone replacement biomaterial but also as bone tissue engineering scaffolds.
Peer Reviewed
Award-winning
Àrees temàtiques de la UPC::Enginyeria biomèdica::Biomaterials
Bone cements
Vertebroplasty
Calcium phosphate cement
Ciments ossis
Fosfat de calci
Vèrtebres -- Cirurgia
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/publishedVersion
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