Microscopic, chemical, and molecular-biological investigation of the decayed medieval stained window glasses of two Catalonian churches

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RECERCAT Principal > Universitat de Barcelona > Mineralogia, Petrologia i Geologia Aplicada > Articles publicats en revistes (Mineralogia, Petrologia i Geologia Aplicada) > Visualizar documento

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dc.contributor	Universitat de Barcelona
dc.contributor.author	Piñar G.
dc.contributor.author	Garcia Vallès, Maite
dc.contributor.author	Gimeno Torrente, Domingo
dc.contributor.author	Fernández Turiel, José Luis
dc.contributor.author	Ettenauer, J.
dc.contributor.author	Sterflinger, K.
dc.date	2013-04-15T17:47:11Z
dc.date	2013-04-15T17:47:11Z
dc.date	2012
dc.date	2013-04-15T17:47:11Z
dc.identifier.citation	0964-8305
dc.identifier.citation	618339
dc.identifier.uri	http://hdl.handle.net/2445/34591
dc.format	13 p.
dc.format	application/pdf
dc.language.iso	eng
dc.publisher	Elsevier B.V.
dc.relation	Versió postprint del document publicat a: 10.1016/j.ibiod.2012.02.008
dc.relation	International Biodeterioration and Biodegradation, 2012, p. 1-13
dc.relation	http://dx.doi.org/10.1016/j.ibiod.2012.02.008
dc.rights	(c) Elsevier B.V., 2012
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	Vitralls
dc.subject	Catalunya
dc.subject	Biodegradació
dc.subject	Glass painting and staining
dc.subject	Catalonia
dc.subject	Biodegradation
dc.title	Microscopic, chemical, and molecular-biological investigation of the decayed medieval stained window glasses of two Catalonian churches
dc.type	info:eu-repo/semantics/article
dc.type	info:eu-repo/semantics/acceptedVersion
dc.description.abstract	We investigated the decayed historical church window glasses of two Catalonian churches, both under Mediterranean climate. Glass surfaces were studied by scanning electron microscopy (SEM), energy dispersive spectrometry (EDS), and X-ray diffraction (XRD). Their chemical composition was determined by avelength-dispersive spectrometry (WDS) microprobe analysis. The biodiversity was investigated by molecular methods: DNA extraction from glass, amplification by PCR targeting the16S rRNA and ITS regions, and fingerprint analyses by denaturing gradient gel electrophoresis (DGGE). Clone libraries containing either PCR fragments of the bacterial 16S rDNA or the fungal ITS regions were screened by DGGE. Clone inserts were sequenced and compared with the EMBL database.

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