Title:
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Influence of electrical stimulation on 3D-cultures of Adipose Tissue Derived Progenitor Cells (ATDPCs) behavior
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Author:
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Castells-Sala, C.; Sánchez Terrones, Benjamín; Recha-Sancho, L.; Puig, V.; Bragós Bardia, Ramon; Semino, C.E.
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Other authors:
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Universitat Politècnica de Catalunya. Departament d'Enginyeria Electrònica; Universitat Politècnica de Catalunya. IEB - Instrumentació Electrònica i Biomèdica |
Abstract:
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Tissue engineering has a fundamental role in regenerative medicine. Still today, the major motivation for cardiac regeneration is to design a platform that enables the complete tissue structure and physiological function regeneration of injured myocardium areas. Although tissue engineering approaches have been generally developed for two-dimensional (2D) culture systems, three-dimensional (3D) systems are being spotlighted as the means to mimic better in vivo cellular conditions. This manuscript examines the influence of electrical stimulation on 3D cultures of adipose tissue-derived progenitor cells (ATDPCs). ATDPCs cells were encapsulated into a self-assembling peptide nanoscaffold (RAD16-I) and continuously electro stimulated during 14-20 days with 2-ms pulses of 50mV/cm at a frequency of 1 Hz. Good cellular network formation and construct diameter reduction was observed in electro stimulated samples. Importantly, the process of electro stimulation does not disrupt cell viability or connectivity. As a future outlook, differentiation studies to cardiomyocytes-like cells will be performed analyzing gene profile and protein expression. |
Abstract:
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Award-winning |
Subject(s):
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-Àrees temàtiques de la UPC::Enginyeria electrònica::Instrumentació i mesura -Àrees temàtiques de la UPC::Enginyeria biomèdica::Electrònica biomèdica -Biomedical engineering -Electric stimulation -Enginyeria biomèdica -Estimulació elèctrica |
Rights:
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Document type:
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Article - Published version Conference Object |
Published by:
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IEEE
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