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Enzymatic degradation of poly(3-hydroxybutyrate) by a commercial lipase
Rodríguez Contreras, Alejandra María; Calafell Monfort, Margarita; Marqués Calvo, M. Soledad
Universitat Politècnica de Catalunya. Departament de Ciència dels Materials i Enginyeria Metal·lúrgica; Universitat Politècnica de Catalunya. Departament d'Òptica i Optometria; Universitat Politècnica de Catalunya. TMAS (T+) - Toxicologia i Microbiologia Ambiental i Sanitària
Polyesters such as poly(3-hydroxybutyrate) (PHB) have attracted commercial and academic interest as new biotechnological materials. In previous studies it was confirmed that two commercial lipases hydrolyzed the ester bonds from the 3HB fractions of P(3HB-co-4HB) copolymer. In this study, one of the previously used commercial lipases has been used for the enzymatic degradation of PHB homopolymer obtained via fermentation with Cupriavidus necator in order to obtain low molecular mass polymer. The results confirmed the enzymatic reaction of the used lipase with this PHB and show a controlled decrease of the molecular mass from 300,000 Da–4000 Da.
Peer Reviewed
Àrees temàtiques de la UPC::Enginyeria dels materials
Polymers
Low molecular weight poly(b-hydroxybutyrate)
Heterogeneous hydrolysis mechanism
Lamellar thickness
Polímers
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/publishedVersion
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