To access the full text documents, please follow this link: http://hdl.handle.net/2445/32319

Grape epicatechin conjugates prevent erythrocyte membrane protein oxidation
Martínez Ocaña, Verónica; Ugartondo Casadevall, Vanessa; Vinardell Martínez-Hidalgo, Ma. Pilar; Torres Simón, Josep Lluís; Mitjans Arnal, Montserrat
Universitat de Barcelona
Epicatechin conjugates obtained from grape have shown antioxidant activity in various systems. However, how these conjugates exert their antioxidant benefits has not been widely studied. We assessed the activity of epicatechin and epicatechin conjugates on the erythrocyte membrane in the presence and absence of a peroxyl radical initiator, to increase our understanding of their mechanisms. Thus, we studied cell membrane fluidity by fluorescence anisotropy measurements, morphology of erythrocytes by scanning electron microscopy, and finally, red cell membrane proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Our data showed that incubation of red cells in the presence of epicatechin derivatives altered membrane fluidity and erythrocyte morphology but not the membrane protein pattern. The presence in the medium of the peroxyl radical initiator 2,2′-azobis(amidinopropane) dihydrochloride (AAPH) resulted in membrane disruptions at all levels analyzed, causing changes in membrane fluidity, cell morphology, and protein degradation. The presence of antioxidants avoided protein oxidation, indicating that the interaction of epicatechin conjugates with the lipid bilayer might reduce the accessibility of AAPH to membranes, which could explain in part the inhibitory ability of these compounds against hemolysis induced by peroxidative insult.
Polifenols
Antioxidants
Raïms
Hematies
Proteïnes de membrana
Polyphenols
Antioxidants
Grapes
Erythrocytes
Membrane proteins
(c) American Chemical Society, Books and Journals Division], 2012
Article
info:eu-repo/semantics/acceptedVersion
American Chemical Society, Books and Journals Division
         

Show full item record

Related documents

Other documents of the same author

Colomer Flores, Aurora; Pinazo Gassol, Aurora; Garcia Ramón, Maria Teresa; Mitjans Arnal, Montserrat; Vinardell Martínez-Hidalgo, Ma. Pilar; Infante Martínez-Pardo, Ma. Rosa; Martínez Ocaña, Verónica; Pérez Muñoz, Lourdes
Morán Badenas, María del Carmen; Mitjans Arnal, Montserrat; Martínez Ocaña, Verónica; Nogueira, Daniele R.; Vinardell Martínez-Hidalgo, Ma. Pilar
Cambras Riu, Trinitat; Anglès Pujolràs, Montserrat; Díez Noguera, Antoni; Martínez Ocaña, Verónica; Moreno Aznárez, Juan José; Mitjans Arnal, Montserrat; Vinardell Martínez-Hidalgo, Ma. Pilar
Cambras Riu, Trinitat; Anglès Pujolràs, Montserrat; Díez Noguera, Antoni; Martínez Ocaña, Verónica; Moreno Aznárez, Juan José; Mitjans Arnal, Montserrat; Vinardell Martínez-Hidalgo, Ma. Pilar
Cambras Riu, Trinitat; Díez Noguera, Antoni; Mitjans Arnal, Montserrat; Moreno Aznárez, Juan José; Martín Venegas, Raquel; Martínez Ocaña, Verónica; Vinardell Martínez-Hidalgo, Ma. Pilar
 

Coordination

 

Supporters