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Transmission electron microscopy in cell biology: sample preparation techniques and image information
Cortadellas i Raméntol, Núria; García, Almudena; Fernández, Eva
Universitat de Barcelona
Transmission electron microscopy is a proven technique in the field of cell biology and a very useful tool in biomedical research. Innovation and improvements in equipment together with the introduction of new technology have allowed us to improve our knowledge of biological tissues, to visualizestructures better and both to identify and to locate molecules. Of all the types ofmicroscopy exploited to date, electron microscopy is the one with the mostadvantageous resolution limit and therefore it is a very efficient technique fordeciphering the cell architecture and relating it to function. This chapter aims toprovide an overview of the most important techniques that we can apply to abiological sample, tissue or cells, to observe it with an electron microscope, fromthe most conventional to the latest generation. Processes and concepts aredefined, and the advantages and disadvantages of each technique are assessedalong with the image and information that we can obtain by using each one ofthem.
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Microscòpia electrònica
Anàlisi instrumental
Microscòpia electrònica de transmissió
Electron microscopy
Instrumental analysis
Transmission electron microscopy
(c) Universitat de Barcelona, 2012
Centres Científics i Tecnològics. Universitat de Barcelona

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