Para acceder a los documentos con el texto completo, por favor, siga el siguiente enlace: http://hdl.handle.net/2445/28883

Membrane-destabilizing activity of pH-responsive cationic lysine-based surfactants: role of charge position and alkyl chain length
Nogueira, Daniele R.; Mitjans Arnal, Montserrat; Morán Badenas, María del Carmen; Pérez Muñoz, Lourdes; Vinardell Martínez-Hidalgo, Ma. Pilar
Universitat de Barcelona
Many strategies for treating diseases require the delivery of drugs into the cell cytoplasm following internalization within endosomal vesicles. Thus, compounds triggered by low pH to disrupt membranes and release endosomal contents into the cytosol are of particular interest. Here, we report novel cationic lysine-based surfactants (hydrochloride salts of N¿- and N¿-acyl lysine methyl ester) that differ in the position of the positive charge and the length of the alkyl chain. Amino acid-based surfactants could be promising novel biomaterials in drug delivery systems, given their biocompatible properties and low cytotoxic potential. We examined their ability to disrupt the cell membrane in a range of pH values, concentrations and incubation times, using a standard hemolysis assay as a model of endosomal membranes. Furthermore, we addressed the mechanism of surfactant-mediated membrane destabilization, including the effects of each surfactant on erythrocyte morphology as a function of pH. We found that only surfactants with the positive charge on the ¿-amino group of lysine showed pH-sensitive hemolytic activity and improved kinetics within the endosomal pH range, indicating that the positive charge position is critical for pH-responsive behavior. Moreover, our results showed that an increase in the alkyl chain length from 14 to 16 carbon atoms was associated with a lower ability to disrupt cell membranes. Knowledge on modulating surfactant-lipid bilayer interactions may help us to develop more efficient biocompatible amino acid-based drug delivery devices.
Sistemes d'administració de medicaments
Aminoàcids
Membranes cel·lulars
Hemòlisi
Agents tensioactius
Drug delivery systems
Amino acids
Cell membranes
Hemolysis
Surface active agents
(c) Springer Verlag, 2011
Artículo
info:eu-repo/semantics/acceptedVersion
Springer Verlag
         

Mostrar el registro completo del ítem

Documentos relacionados

Otros documentos del mismo autor/a

Nogueira, Daniele R.; Morán Badenas, María del Carmen; Mitjans Arnal, Montserrat; Pérez Muñoz, Lourdes; Ramos, David; Lapuente Pérez, Joaquín de; Vinardell Martínez-Hidalgo, Ma. Pilar
Nogueira, Daniele R.; Morán Badenas, María del Carmen; Mitjans Arnal, Montserrat; Martínez Ocaña, Verónica; Pérez Muñoz, Lourdes; Vinardell Martínez-Hidalgo, Ma. Pilar
Morán Badenas, María del Carmen; Mitjans Arnal, Montserrat; Martínez Ocaña, Verónica; Nogueira, Daniele R.; Vinardell Martínez-Hidalgo, Ma. Pilar
Nogueira, Daniele R.; Mitjans Arnal, Montserrat; Busquets i Viñas Ma. Antonia; Pérez Muñoz, Lourdes; Vinardell Martínez-Hidalgo, Ma. Pilar
Nogueira, Daniele R.; Tavano, Lorena; Mitjans Arnal, Montserrat; Pérez Muñoz, Lourdes; Infante Martínez-Pardo, Ma. Rosa; Vinardell Martínez-Hidalgo, Ma. Pilar