To access the full text documents, please follow this link: http://hdl.handle.net/2445/25228

Calmodulin Regulates Intracellular Trafficking of Epidermal Growth Factor Receptor and the MAPK Signaling Pathway
Tebar Ramon, Francesc; Vilallonga Smith, Príam de; Sorkina, Tatiana; Agell i Jané, Neus; Sorkin, Alexander; Enrich Bastús, Carles
Universitat de Barcelona
The epidermal growth factor receptor (EGFR) is a member of the tyrosine kinase receptor family involved in signal transduction and the regulation of cellular proliferation and differentiation. It is also a calmodulin-binding protein. To examine the role of calmodulin in the regulation of EGFR, the effect of calmodulin antagonist, W-13, on the intracellular trafficking of EGFR and the MAPK signaling pathway was analyzed. W-13 did not alter the internalization of EGFR but inhibited its recycling and degradation, thus causing the accumulation of EGF and EGFR in enlarged early endosomal structures. In addition, we demonstrated that W-13 stimulated the tyrosine phosphorylation of EGFR and consequent recruitment of Shc adaptor protein with EGFR, presumably through inhibition of the calmodulin-dependent protein kinase II (CaM kinase II). W-13¿mediated EGFR phosphorylation was blocked by metalloprotease inhibitor, BB94, indicating a possible involvement of shedding in this process. However, MAPK activity was decreased by W-13; dissection of this signaling pathway showed that W-13 specifically interferes with Raf-1 activity. These data are consistent with the regulation of EGFR by calmodulin at several steps of the receptor signaling and trafficking pathways.
Calmodulina
Transducció de senyal cel·lular
Factor de creixement epidèrmic
Calmodulin
Cellular signal transduction
Epidermal growth factor
cc-by-nc-sa, (c) Tebar et al., 2002
http://creativecommons.org/licenses/cc-by-nc-sa/3.0/es
Article
info:eu-repo/semantics/publishedVersion
American Society for Cell Biology
         

Show full item record

Related documents

Other documents of the same author

Lladó, Anna; Tebar Ramon, Francesc; Calvo Ademuz, Maria; Moretó Elias, Jemina; Sorkin, Alexander; Enrich Bastús, Carles
Lu, Albert; Tebar Ramon, Francesc; Alvarez-Moya, Blanca; López Alcalá, Cristina; Calvo Ademuz, Maria; Enrich Bastús, Carles; Agell i Jané, Neus; Nakamura, Takeshi; Matsuda, Michiyuki; Bachs Valldeneu, Oriol
Barceló, Carles; Paco, Noelia; Beckett. Alison J.; Alvarez-Moya, Blanca; Garrido, Eduard; Gelabert, Mariona; Tebar Ramon, Francesc; Jaumot i Pijoan, Montserrat; Prior, Ian; Agell i Jané, Neus
Lladó, Anna; Timpson, Paul; Vilà de Muga, Sandra; Moretó Elias, Jemina; Pol i Sorolla, Albert; Grewal, Thomas; Daly, Roger J.; Enrich Bastús, Carles; Tebar Ramon, Francesc
 

Coordination

 

Supporters