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dc.contributor | Universitat de Barcelona |
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dc.contributor.author | Alcaraz Casademunt, Jordi |
dc.contributor.author | Buscemi Estefanell, Lara |
dc.contributor.author | Grabulosa Descals, Mireia |
dc.contributor.author | Trepat Guixer, Xavier |
dc.contributor.author | Fabry, Ben |
dc.contributor.author | Farré Ventura, Ramon |
dc.contributor.author | Navajas Navarro, Daniel |
dc.date | Lung epithelial cells are subjected to large cyclic forces from breathing. However, their response to dynamic stresses is poorly defined. We measured the complex shear modulus ( G * ( ω )) of human alveolar (A549) and bronchial (BEAS-2B) epithelial cells over three frequency decades (0.1–100Hz) and at different loading forces (0.1–0.9nN) with atomic force microscopy. G * ( ω ) was computed by correcting force-indentation oscillatory data for the tip-cell contact geometry and for the hydrodynamic viscous drag. Both cell types displayed similar viscoelastic properties. The storage modulus G ′( ω ) increased with frequency following a power law with exponent ∼0.2. The loss modulus G ″( ω ) was ∼2/3 lower and increased similarly to G ′( ω ) up to ∼10Hz, but exhibited a steeper rise at higher frequencies. The cells showed a weak force dependence of G ′( ω ) and G ″( ω ). G * ( ω ) conformed to the power-law model with a structural damping coefficient of ∼0.3, indicating a coupling of elastic and dissipative processes within the cell. Power-law behavior implies a continuum distribution of stress relaxation time constants. This complex dynamics is consistent with the rheology of soft glassy materials close to a glass transition, thereby suggesting that structural disorder and metastability may be fundamental features of cell architecture. |
dc.date | 2012-05-14T14:12:23Z |
dc.date | 2012-05-14T14:12:23Z |
dc.date | 2003 |
dc.identifier.citation | 0006-3495 |
dc.identifier.citation | 504995 |
dc.identifier.uri | http://hdl.handle.net/2445/25622 |
dc.format | 9 p. |
dc.format | application/pdf |
dc.language.iso | eng |
dc.publisher | Biophysical Society |
dc.relation | Reproducció del document publicat a: http://dx.doi.org/10.1016/S0006-3495(03)75014-0 |
dc.relation | Biophysical Journal, 2003, vol. 84, num. 3, p. 2071-2079 |
dc.relation | http://dx.doi.org/10.1016/S0006-3495(03)75014-0 |
dc.rights | (c) Biophysical Society, 2003 |
dc.rights | info:eu-repo/semantics/openAccess |
dc.subject | Microscòpia de força atòmica |
dc.subject | Membrana mucosa |
dc.subject | Reologia (Biologia) |
dc.subject | Atomic force microscopy |
dc.subject | Mucous membrane |
dc.subject | Rheology (Biology) |
dc.title | Microrheology of human lung epithelial cells measured by atomic force microscopy |
dc.type | info:eu-repo/semantics/article |
dc.type | info:eu-repo/semantics/publishedVersion |
dc.description.abstract |