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Detection, properties, and frequency of local calcium release from the sarcoplasmic reticulum in teleost cardiomyocytes
Llach, Anna; Molina, Cristina E.; Álvarez Lacalle, Enrique; Tort, Lluis; Benítez Iglesias, Raúl; Hove-Madsen, Leif
Universitat Politècnica de Catalunya. Departament de Física Aplicada; Universitat Politècnica de Catalunya. Departament d'Enginyeria de Sistemes, Automàtica i Informàtica Industrial; Universitat Politècnica de Catalunya. NOLIN - Física No-Lineal i Sistemes Fora de l'Equilibri
Calcium release from the sarcoplasmic reticulum (SR) plays a central role in the regulation of cardiac contraction and rhythm in mammals and humans but its role is controversial in teleosts. Since the zebrafish is an emerging model for studies of cardiovascular function and regeneration we here sought to determine if basic features of SR calcium release are phylogenetically conserved. Confocal calcium imaging was used to detect spontaneous calcium release (calcium sparks and waves) from the SR. Calcium sparks were detected in 16 of 38 trout atrial myocytes and 6 of 15 ventricular cells. The spark amplitude was 1.4560.03 times the baseline fluorescence and the time to half maximal decay of sparks was 2763 ms. Spark frequency was 0.88 sparks mm21 min21 while calcium waves were 8.5 times less frequent. Inhibition of SR calcium uptake reduced the calcium transient (F/F0) from 1.7760.17 to 1.1260.18 (p=0.002) and abolished calcium sparks and waves. Moreover, elevation of extracellular calcium from 2 to 10 mM promoted early and delayed afterdepolarizations (from 0.660.3 min21 to 8.162.0 min21, p = 0.001), demonstrating the ability of SR calcium release to induce afterdepolarizations in the trout heart. Calcium sparks of similar width and duration were also observed in zebrafish ventricular myocytes. In conclusion, this is the first study to consistently report calcium sparks in teleosts and demonstrate that the basic features of calcium release through the ryanodine receptor are conserved, suggesting that teleost cardiac myocytes is a relevant model to study the functional impact of abnormal SR function.
Peer Reviewed
Àrees temàtiques de la UPC::Enginyeria biomèdica::Electrònica biomèdica
Osteichthyes -- Cardiovascular system
Cardiovascular Diseases -- diagnosis
Osteïctis -- Sistema cardiovascular
Malalties cardiovasculars -- Diagnòstic

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