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Title:
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Multisite recording of extracellular potentials produced by microchannel-confined neurons in-vitro
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Author:
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Claverol Tinturé, Enrique; Cabestany Moncusí, Joan; Rosell Ferrer, Francisco Javier
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Other authors:
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Universitat Politècnica de Catalunya. Departament d'Enginyeria Electrònica; Universitat Politècnica de Catalunya. IEB - Instrumentació Electrònica i Biomèdica |
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Abstract:
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Towards establishing electrical interfaces with patterned in vitro neurons, we have previously described the fabrication of hybrid elastomer-glass devices polymer-on-multielectrode array technology and obtained single-electrode recordings of extracellular potentials from confined neurons (Claverol-Tinturé et al., 2005). Here, we demonstrate the feasibility of spatially localized multisite recordings from individual microchannel-guided neurites extending from microwell-confined somas with good signal-to-noise ratios (20 dB) and spike magnitudes of up to 300 μV.
Single-cell current source density (scCSD) analysis of the spatio-temporal
patterns of membrane currents along individual processes is illustrated. |
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Abstract:
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Peer Reviewed |
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Subject(s):
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Àrees temàtiques de la UPC::Enginyeria biomèdica::Electrònica biomèdica
Electrodiagnosis
Membranes (Biology)
Biolectric potentials
Biomembranes
BioMEMS
Cellular biophysics
Microelectrodes
Neurophysiology
Spatiotemporal phenomena
Multisite recording
Extracellular potentials
Microchannel-confined neurons
Electrical interfaces
Patterned in vitro neurons
Hybrid elastomer-glass. devices polimer-on-multielectrode array technology
Single-electrode recordings
Spatially localized multisite recordings
Microchannel-guided neurites
Microwell-confined somas
Single-cell current source density
Spatiotemporal patterns
Membrane currents
Electrodiagnòstic
Membranes (Biologia) |
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Rights:
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Document type:
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Article |
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Published by:
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IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC
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