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Whole-rat protein content estimation: applicability of the Nx6.25 factor
Rafecas Jorba, Immaculada; Esteve, M.; Fernández López, José Antonio; Remesar Betlloch, Xavier; Alemany, Marià, 1946-
Universitat de Barcelona
The amino acid composition of the protein from three strains of rat (Wistar, Zucker lean and Zucker obese), subjected to reference and high-fat diets has been used to determine the mean empirical formula, molecular weight and N content of whole-rat protein. The combined whole protein of the rat was uniform for the six experimental groups, containing an estimate of 17.3% N and a mean aminoacyl residue molecular weight of 103.7. This suggests that the appropriate protein factor for the calculation of rat protein from its N content should be 5.77 instead of the classical 6.25. In addition, an estimate of the size of the non-protein N mass in the whole rat gave a figure in the range of 5.5 % of all N. The combination of the two calculations gives a protein factor of 5.5 for the conversion of total N into rat protein.
02-03-2012
Rates (Animals de laboratori)
Proteïnes dels aliments
Nitrogen conversion factor (6.25)
Protein analysis
Protein nitrogen
Rat
(c) The Nutrition Society, 1994
Artículo
The Nutrition Society, 1994
         

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