Para acceder a los documentos con el texto completo, por favor, siga el siguiente enlace:

Pressure-jump-induced kinetics reveals a hydration dependent folding/unfolding mechanism of ribonuclease A
Font Sentias, Josep; Torrent i Mas, Joan; Ribó i Panosa, Marc; Laurents, Douglas V.; Balny, Claude; Vilanova i Brugués, Maria; Lange, Reinhard
Pressure-jump (p-jump)-induced relaxation kinetics was used to explore the energy landscape of protein folding/unfolding of Y115W, a fluorescent variant of ribonuclease A. Pressure-jumps of 40MPa amplitude (5ms dead-time) were conducted both to higher (unfolding) and to lower (folding) pressure, in the range from 100 to 500MPa, between 30 and 50°C. Significant deviations from the expected symmetrical protein relaxation kinetics were observed. Whereas downward p-jumps resulted always in single exponential kinetics, the kinetics induced by upward p-jumps were biphasic in the low pressure range and monophasic at higher pressures. The relative amplitude of the slow phase decreased as a function of both pressure and temperature. At 50°C, only the fast phase remained. These results can be interpreted within the framework of a two-dimensional energy surface containing a pressure- and temperature-dependent barrier between two unfolded states differing in the isomeric state of the Asn-113–Pro-114 bond. Analysis of the activation volume of the fast kinetic phase revealed a temperature-dependent shift of the unfolding transition state to a larger volume. The observed compensation of this effect by glycerol offers an explanation for its protein stabilizing effect
Tots els drets reservats
Biophysical Society

Mostrar el registro completo del ítem

Documentos relacionados

Otros documentos del mismo autor/a

Torrent i Mas, Joan; Marchal, Stéphane; Ribó i Panosa, Marc; Vilanova i Brugués, Maria; Georges, Cédric; Dupont, Yves; Lange, Reinhard
Callís Figueres, Mariona; Serrano, Soraya; Benito i Mundet, Antoni; Laurents, Douglas V.; Vilanova i Brugués, Maria; Bruix, Marta; Ribó i Panosa, Marc
Castro Gallegos, Jessica; Ribó i Panosa, Marc; Navarro, Susanna; Nogués, Maria Victòria; Vilanova i Brugués, Maria; Benito i Mundet, Antoni
Vert Company, Anna; Castro Gallegos, Jessica; Ribó i Panosa, Marc; Benito i Mundet, Antoni; Vilanova i Brugués, Maria
Vert Company, Anna; Castro Gallegos, Jessica; Ribó i Panosa, Marc; Benito i Mundet, Antoni; Vilanova i Brugués, Maria