Para acceder a los documentos con el texto completo, por favor, siga el siguiente enlace: http://hdl.handle.net/2445/18673

Anti-inflammatory effects of pancreatitis associated protein in inflammatory bowel disease
Gironella Cos, Meritxell; Iovanna, J. L.; Sans i Cuffí, Miquel; Gil, F.; Peñalva, M.; Closa i Autet, Daniel, 1961-; Miquel Morera, Rosa; Piqué, J. M. (Piqué Badía); Panés Díaz, Julià
Universitat de Barcelona
Background and aims: Increased pancreatitis associated protein (PAP) mRNA has been reported in active inflammatory bowel disease (IBD). The aims of the current study were to characterise PAP production in IBD and the effects of PAP on inflammation. Patients and methods: Serum PAP levels were determined in healthy controls (n¿=¿29), inflammatory controls (n¿=¿14), and IBD patients (n¿=¿171). Ex vivo PAP secretion in intestinal tissue was measured in 56 IBD patients and 13 healthy controls. Cellular origin of PAP was determined by immunohistochemistry. The effects of exogenous PAP on nuclear factor ¿B (NF¿B) activation, proinflammatory cytokine production, and endothelial adhesion molecule expression were also analysed ex vivo. Results: Patients with active IBD had increased serum PAP levels compared with controls, and these levels correlated with clinical and endoscopic disease severity. Ex vivo intestinal PAP synthesis was increased in active IBD and correlated with endoscopic and histological severity of inflammatory lesions. PAP localised to colonic Paneth cells. Incubation of mucosa from active Crohn¿s disease with PAP dose dependently reduced proinflammatory cytokines secretion. PAP prevented TNF-¿ induced NF¿B activation in monocytic, epithelial, and endothelial cells and reduced proinflammatory cytokine mRNA levels and adhesion molecule expression. Conclusions: PAP is synthesised by Paneth cells and is overexpressed in colonic tissue of active IBD. PAP inhibits NF¿B activation and downregulates cytokine production and adhesion molecule expression in inflamed tissue. It may represent an anti-inflammatory mechanism and new therapeutic strategy in IBD.
26-07-2011
Malalties inflamatòries intestinals
Pancreatitis
Proteïnes
Inflammatory bowel diseases
Pancreatitis
Proteins
(c) BMJ Publishing Group Ltd and British Society of Gastroenterology, 2005
Artículo
BMJ Group
         

Mostrar el registro completo del ítem

Documentos relacionados

Otros documentos del mismo autor/a

Salas Martínez, Azucena; Sans i Cuffí, Miquel; Soriano Viladomiu, Alex; Reverter Calatayud, Juan Carlos; Anderson, D. C.; Piqué, J. M. (Piqué Badía); Panés Díaz, Julià
Adán Liébana, Cristina; Ardévol Grau, Anna; Closa i Autet, Daniel, 1961-; Remesar Betlloch, Xavier; Alemany, Marià, 1946-; Fernández López, José Antonio
Sáiz Calahorra, Juan Carlos; Sans i Cuffí, Miquel; Mas Comas, Ana M.; Olmedo Casas, Eva; Forns, Xavier; López Labrador, Francesc Xavier; Restrepo Gutiérrez, Juan Carlos; Costa i Camps, Josep; Salmerón Bargo, Juan Manuel; Guilera Sardà, Magda; Ampurdanés, Sergi; Sánchez Tapias, José M. (José María); Jiménez de Anta Losada, María Teresa; Rodés, J.
Tailor, A.; Tomlinson, Annette, 1942-; Salas Martínez, Azucena; Panés Díaz, Julià; Granger, D. Neil; Flower, Roderick J.; Perretti, M.
Rimola, J.; Rodríguez, S.; García Bosch, O.; Ordás, I.; Ayala, E.; Aceituno, M.; Pellisé Urquiza, Maria; Ayuso Colella, Carmen; Ricart, Elena; Donoso Bach, Lluís; Panés Díaz, Julià