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dc.contributor.author | de la Fuente Oliver, Gabriel |
---|---|
dc.contributor.author | Cebrián, J. A. |
dc.contributor.author | Fondevila, Manuel |
dc.date | 2015-12-02T16:23:12Z |
dc.date | 2006 |
dc.date | 2015-12-02T16:23:12Z |
dc.date | 10000-01-01 |
dc.identifier | 0266-8254 |
dc.identifier | http://hdl.handle.net/10459.1/49220 |
dc.identifier | https://doi.org/10.1111/j.1472-765X.2006.01894.x |
dc.identifier.uri | http://hdl.handle.net/10459.1/49220 |
dc.description | Aims: To study the viability of Diploplastron (Metadinium) affine after its cryopreservation at two cooling rates, and the effect of procedure conditions on viability. Methods and Results: There were differences in viability between cooling rates (1 and 4°C min−1) at 15 or 5°C, but not after thawing. When the equilibrium temperature (25 or 5°C), the cryopreservant (glycerol or dimethyl sulfoxide [DMSO]) and the use of membrane protector were tested, there were no differences caused by the cryopreservant or the membrane protector. However, the equilibrium at 25°C increased the viability (P = 0·005) compared with 5°C. Conclusions: Viability after thawing was 0·10 0·19. Adding the cryopreservant (either glycerol or DMSO) at 25°C instead of 5°C improves viability of D. affine after thawing. Significance and Impact of the Study: Conditions of cryopreservation are largely dependent on the species of rumen protozoa. Number of viable cells after thawing would indicate the possibility of culture recovery for D. affine. |
dc.description | This work has been financed by the project AGL2004-02910. Thanks are given to Prof B.A. Dehority for hiscritical review of the manuscript. |
dc.format | application/pdf |
dc.language | eng |
dc.publisher | Blackwell Science |
dc.relation | MIECI/PN2004-2007/AGL2004-02910 |
dc.relation | Reproducció del document publicat a:https://doi.org/10.1111/j.1472-765X.2006.01894.x |
dc.relation | Letters in Applied Microbiology, 2006, vol.42, p. 573-577 |
dc.rights | (c) Blackwell Science, 2006 |
dc.rights | info:eu-repo/semantics/restrictedAccess |
dc.subject | Remugants |
dc.subject | Protozous |
dc.subject | Criobiologia |
dc.subject | Ruminants |
dc.subject | Protozoa |
dc.subject | Cryobiology |
dc.title | Effect of the cryopreservation conditions on the viability of the rumen ciliate Diploplastron (Metadinium) affine |
dc.type | info:eu-repo/semantics/article |
dc.type | info:eu-repo/semantics/publishedVersion |
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023090.pdf | 124.6 KB | application/pdf | Visualitza/Obre |