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Título:
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Comparative cytogenetic study of three Macrolophus species (Heteroptera, Miridae.)
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Autor/a:
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Jauset Berrocal, Ana Maria; Edo-Tena, Eva; Castañé Fernández, Cristina; Agustí Abella, Núria; Alomar, Òscar; Grozeva, S.
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Notas:
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Macrolophus pygmaeus (Rambur, 1839) (Insecta, Heteroptera, Miridae) is a predator of key vegetable crop pests applied as a biocontrol agent in the Mediterranean region. M. pygmaeus and M. melanotoma (A. Costa, 1853) are cryptic species with great morphological similarity which results in their misidentification and negative consequences for the conservation of their populations on greenhouse and outdoor crops. In order to find out specific markers for their separation we studied the karyotype, male meiosis and heterochromatin composition of these species and additionally of a third species (as a reference one), M. costalis Fieber, 1858. We demonstrate here that all the three species share achiasmate male meiosis and sex chromosome pre-reduction. On the other hand, the species differ in karyotype, with 2n=28 (26+XY) in M. pygmaeus, 2n=27 (24+X1X2Y) in M. costalis, and 2n=34 (32+XY) in M. melanotoma, and heterochromatin distribution and composition. In addition, the species differ in sperm morphology: sperm cells of M. costalis are significantly longer with longer head and tail than those of M. melanotoma and M. pygmaeus, whereas sperm cells of M. melanotoma have a longer tail than those of M. pygmaeus. All these characters can be used as markers to identify the species, in particular the cryptic species M. melanotoma and M. pygmaeus.
This study has been funded by the Spanish Ministry of Economy and Competitiveness
(MINECO) (Project AGL2011-24349), and by a travel grant from the University of
Lleida - Fundació “La Caixa” to Dr Grozeva. The chromosome analysis was performed
using microscope Axio Scope A1 – Carl Zeiss Microscopy upgraded by the project
WETLANET (FP7 CSA – SUPPORT ACTION, GA 229802). We thank cordially
Prof. Dr V. Kuznetsova for the valuable advices to improve the manuscript |
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Materia(s):
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-Macrolophus
-Miridae
-Karyotype
-sperm morphology
-achiasmate meiosis
-Control biològic de plagues
-Mediterrània (Regió)
-Hemípters
-Citogenètica
-Biological pest control
-Mediterranean Region
-Hemiptera
-Cytogenetics |
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Derechos:
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cc-by (c) Jauset et al., 2015
http://creativecommons.org/licenses/by/4.0/
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Tipo de documento:
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Artículo
Artículo - Versión publicada |
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Editor:
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Pensoft Publishers
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Compartir:
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