Title:
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Osmotic stress causes a G1 cell cycle delay and downregulation of Cln3/Cdc28 activity in Saccharomyces cerevisiae
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Author:
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Bellí i Martínez, Gemma; Garí Marsol, Eloi; Aldea, Martí; Herrero Perpiñán, Enrique
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Notes:
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Moderate hyperosmotic stress on Saccharomyces
cerevisiae cells produces a temporary delay at the G1
stage of the cell cycle. This is accompanied by
transitory downregulation of CLN1, CLN2 and CLB5
transcript levels, although not of CLN3, which codes
for the most upstream activator of the G1/S transition.
Osmotic shock to cells synchronized in early G1,
when Cln3 is the only cyclin present, causes a delay
in cell cycle resumption. This points to Cln3 as being
a key cell cycle target for osmotic stress. We have
observed that osmotic shock causes downregulation
of the kinase activity of Cln3±Cdc28 complexes. This
is concomitant with a temporary accumulation of
Cln3 protein as a result of increased stability. The
effects of the osmotic stress in G1 are not suppressed
in CLN3-1 cells with increased kinase activity, as the
Cln3±Cdc28 activity in this mutant is still affected by
the shock. Although Hog1 is not required for the
observed cell cycle arrest in hyperosmotic conditions,
it is necessary to resume the cell cycle at KCl
concentrations higher than 0.4 M. |
Rights:
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(c) Blackwell Science, 2001
info:eu-repo/semantics/restrictedAccess |
Document type:
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article publishedVersion |
Published by:
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Blackwell Science
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