Para acceder a los documentos con el texto completo, por favor, siga el siguiente enlace:

Wound response in orange as a resistance mechanism against Penicillium digitatum (pathogen) and P. expansum (non-host pathogen)
Vilanova, Laura; Torres Sanchis, Rosario; Viñas Almenar, Inmaculada; González-Candelas, Luis; Usall i Rodié, Josep; Fiori, S.; Solsona Aixalà, Cristina; Teixidó i Espasa, Neus
Penicillium digitatum is the most devastating postharvest pathogen of citrus. In addition, Penicillium expansum is the main pathogen of pome fruit, although recent studies have demonstrated its ability to infect oranges under some conditions. In this study, we evaluated wound response in"Valencia" oranges harvested at three different maturity stages and the effect of wound response on the establishment of both pathogens when fruit were stored at two different temperatures (20 and 4 °C). The effect of wounding and pathogen inoculation on lignin content, was also quantified. Lastly, the expression of several phenylpropanoid pathway-related genes was also analyzed by semi-quantitative RT-PCR. Results indicated that, in general, P. digitatum exhibited lower decay incidence and severity as time between wounding and inoculation increased. Decay incidence and severity were higher in fruit from the over-mature harvest than in fruit from immature and commercial harvests. P. expansum was able to infect fruit at 20 °C but lesions were small compared to lesion size of fruit stored at 4 °C. Lignin content in wounded fruit (control) and in samples wounded and inoculated with P. expansum was highest in fruit from the immature harvest at 7 d post-wounding and inoculation. Wounded fruit had higher expression of pal1, comt1 and pox1 genes at 48 h than at 24 h. However, samples inoculated with P. digitatum showed lower expression at 48 h than at 24 h. Our results indicated that maturity and storage temperature play an important role in orange wound response.
Green mould
Blue mould
Maturity stage
Wound response
(c) Elsevier, 2013

Documentos con el texto completo de este documento

Ficheros Tamaño Formato Vista
022616.pdf 788.0 KB application/pdf Vista/Abrir

Mostrar el registro completo del ítem

Documentos relacionados

Otros documentos del mismo autor/a