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Títol:
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Pkc1 and the upstream elements of the cell integrity pathway in Saccharomyces cerevisiae, Rom2 and Mtl1, are required for cellular responses to oxidative stress
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Autor/a:
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Vilella Mitjana, Felipe; Herrero Perpiñán, Enrique; Torres Rosell, Jordi; Torre Ruiz, M. A. de la
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Notes:
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In this study we analyze the participation of the
PKC1-MAPK cell integrity pathway in cellular re-
sponses to oxidative stress in Saccharomyces cerevisiae.
Evidence is presented demonstrating that only Pkc1
and the upstream elements of the cell integrity pathway
are essential for cell survival upon treatment with two
oxidizing agents, diamide and hydrogen peroxide. Mtl1
is characterized for the first time as a cell-wall sensor of
oxidative stress. We also show that the actin cytoskele-
ton is a cellular target for oxidative stress. Both diamide
and hydrogen peroxide provoke a marked depolariza-
tion of the actin cytoskeleton, being Mtl1, Rom2 and
Pkc1 functions all required to restore the correct actin
organization. Diamide induces the formation of disul-
fide bonds in newly secreted cell-wall proteins. This
mainly provokes structural changes in the cell outer
layer, which activate the PKC1-MAPK pathway and
hence the protein kinase Slt2. Our results led us to the
conclusion that Pkc1 activity is required to overcome
the effects of oxidative stress by: (i) enhancing the ma-
chinery required to repair the altered cell wall and (ii)
restoring actin cytoskeleton polarity by promoting actin
cable formation. |
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Drets:
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(c) The American Society for Biochemistry and Molecular Biology, 2005
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Tipus de document:
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article
publishedVersion |
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Publicat per:
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The American Society for Biochemistry and Molecular Biology
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