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Título:
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Amphotericin B mediates killing in Cryptococcus neoformans through the induction of a strong oxidative burst
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Autor/a:
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Sangalli-Leite, Fernanda; Scorzoni, Liliana; Mesa-Arango, Ana Cecilia; Casas Herranz, Celia; Herrero Perpiñán, Enrique; Mendes Gianinni, Maria José Soares; Rodríguez-Tudela, Juan Luis; Cuenca-Estrella, Manuel; Zaragoza, Oscar
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Notas:
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We studied the effects of Amphotericin B (AmB) on Cryptococcus neoformans using different viability methods (CFUs enumeration, XTT
assay and propidium iodide permeability). After 1 h of incubation, there were no viable colonies when the cells were exposed to AmB concentrations ≥ 1 mg/L. In the same conditions, the cells did not become permeable to propidium iodide, a phenomenon that was not observed
until 3 h of incubation. When viability was measured in parallel using XTT assay, a result consistent with the CFUs was obtained, although we
also observed a paradoxical effect in which at high AmB concentrations, a higher XTT reduction was measured than at intermediate AmB
concentrations. This paradoxical effect was not observed after 3 h of incubation with AmB, and lack of XTT reduction was observed at AmB
concentrations higher than 1 mg/L. When stained with dihydrofluorescein, AmB induced a strong intracellular oxidative burst. Consistent with
oxidative damage, AmB induced protein carbonylation. Our results indicate that in C. neoformans, Amphotericin B causes intracellular damage mediated through the production of free radicals before damage on the cell membrane, measured by propidium iodide uptake. |
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Materia(s):
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-Cryptococcus neoformans
-Amphotericin B
-Oxidative burst |
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Derechos:
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(c) Elsevier, 2011
(c) Institut Pasteur, 2011
info:eu-repo/semantics/restrictedAccess |
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Tipo de documento:
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article
publishedVersion |
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Editor:
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Elsevier
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Compartir:
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