dc.contributor.author |
Pedroso, Nuno |
dc.contributor.author |
Matias, Ana C. |
dc.contributor.author |
Cyrne, Luísa |
dc.contributor.author |
Antunes, Fernando |
dc.contributor.author |
Borges, Carlos |
dc.contributor.author |
Malhó, Rui |
dc.contributor.author |
de Almeida, Rodrigo F.M. |
dc.contributor.author |
Herrero Perpiñán, Enrique |
dc.contributor.author |
Marinho, H. Susana |
dc.date |
2015-07-07T11:18:48Z |
dc.date |
2009 |
dc.date |
10000-01-01 |
dc.identifier |
0891-5849 |
dc.identifier |
http://hdl.handle.net/10459.1/48423 |
dc.identifier |
https://doi.org/10.1016/j.freeradbiomed.2008.10.039 |
dc.identifier.uri |
http://hdl.handle.net/10459.1/48423 |
dc.description |
In Saccharomyces cerevisiae, the rate of hydrogen peroxide (H2O2) diffusion through the plasma membrane
decreases during adaptation to H2O2 by a still unknown mechanism. Here, adaptation to H2O2 was observed
to modulate rapidly the expression of genes coding for enzymes involved in ergosterol and lipid metabolism.
Adaptation to H2O2 also alters plasma membrane lipid composition. The main changes were the following:
(a) there was a decrease in oleic acid (30k) and in the ratio between unsaturated and saturated long-chain
fatty acids; (b) the phosphatidylcholine:phosphatidylethanolamine ratio increased threefold; (c) sterol levels
were unaltered but there was an increased heterogeneity of sterol-rich microdomains and increased ordered
domains; (d) the levels of the sterol precursor squalene increased twofold, in agreement with ERG1 gene
down-regulation; and (e) C26:0 became the major very long chain fatty acid owing to an 80k decrease in 2-
hydroxy-C26:0 levels and a 50k decrease in C20:0 levels, probably related to the down-regulation of fatty
acid elongation (FAS1, FEN1, SUR4) and ceramide synthase (LIP1, LAC1) genes. Therefore, H2O2 leads to a
reorganization of the plasma membrane microdomains, which may explain the lower permeability to H2O2,
and emerges as an important regulator of lipid metabolism and plasma membrane lipid composition. |
dc.language |
eng |
dc.publisher |
Elsevier |
dc.relation |
Reproducció del document publicat a https://doi.org/10.1016/j.freeradbiomed.2008.10.039 |
dc.relation |
Free Radical Biology & Medicine, 2009, vol. 46, núm. 2, p. 289-298 |
dc.rights |
(c) Elsevier, 2008 |
dc.rights |
info:eu-repo/semantics/restrictedAccess |
dc.subject |
H2O2 adaptation |
dc.subject |
Plasma membrane permeability |
dc.subject |
Very long chain fatty acids |
dc.title |
Modulation of plasma membrane lipid profile and microdomains by H2O2 in Saccharomyces cerevisiae |
dc.type |
article |
dc.type |
publishedVersion |