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Título:
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Down-regulation of fatty acid synthase increases the resistance of Saccharomyces cerevisiae cells to H2O2
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Autor/a:
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Matias, Ana C.; Pedroso, Nuno; Teodoro, Nuno; Marinho, H. Susana; Antunes, Fernando; Nogueira, José Manuel; Herrero Perpiñán, Enrique; Cyrne, Luísa
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Notas:
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Changes in plasma membrane permeability caused by H2O2 were recently found to be involved in the adaptation to H2O2, but the mechanism
responsible for this change remains largely unknown. Here this mechanism was addressed and two lines of evidence showed for the first time that
fatty acid synthase (Fas) plays a key role during the cellular response of Saccharomyces cerevisiae to H2O2: (1) adaptation was associated with a
decrease in both Fas expression and activity; (2) more importantly, decreasing Fas activity by 50% through deletion of one of the FAS alleles
increased the resistance to lethal doses of H2O2. The mechanism by which a decrease of Fas expression causes a higher resistance to H2O2 was not
fully elucidated. However, the fas1Δ strain plasma membrane had large increases in the levels of lignoceric acid (C24:0) (40%) and cerotic acid
(C26:0) (50%), suggesting that alterations in the plasma membrane composition are involved. Very-long-chain fatty acids (VLCFA) through
interdigitation or by modulating formation of lipid rafts may decrease the overall or localized plasma membrane permeability to H2O2, respectively,
thus conferring a higher resistance to H2O2. |
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Materia(s):
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-H2O2 adaptation
-Fatty acid synthase
-Very-long-chain fatty acids |
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Derechos:
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(c) Elsevier, 2007
info:eu-repo/semantics/restrictedAccess |
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Tipo de documento:
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article
publishedVersion |
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Editor:
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Elsevier
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Compartir:
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