dc.contributor.author |
Cabiscol Català, Elisa |
dc.contributor.author |
Bellí i Martínez, Gemma |
dc.contributor.author |
Tamarit Sumalla, Jordi |
dc.contributor.author |
Echave Lozano, Pedro |
dc.contributor.author |
Herrero Perpiñán, Enrique |
dc.contributor.author |
Ros Salvador, Joaquim |
dc.date |
2015-07-02T09:34:39Z |
dc.date |
2002 |
dc.identifier |
0021-9258 |
dc.identifier |
http://hdl.handle.net/10459.1/48399 |
dc.identifier |
https://doi.org/10.1074/jbc.M206525200 |
dc.identifier.uri |
http://hdl.handle.net/10459.1/48399 |
dc.description |
In the present study, we have analyzed the role of the
molecular chaperone Hsp60 in protection of Saccharo-
myces cerevisiae against oxidative damage. We con-
structed mutant strains in which the levels of Hsp60
protein, compared with wild-type cells, were four times
greater, and the addition of doxycycline gradually re-
duces them to 20% of wild-type. Under oxidative-stress
conditions, the progressive decrease in Hsp60 levels in
these mutants resulted in reduced cell viability and an
increase in both cell peroxide species and protein car-
bonyl content. Protection of Fe/S-containing enzymes
from oxidative inactivation was found to be dose-de-
pendent with respect to Hsp60 levels. As these enzymes
release their iron ions under oxidative-stress condi-
tions, the intracellular labile iron pool, monitored with
calcein, was higher in cells with reduced Hsp60 levels.
Consistently, the iron chelator deferoxamine protected
low Hsp60-expressing cells from both oxidant-induced
death and protein oxidation. These results indicate that
the role of Hsp60 in oxidative-stress defense is explained
by protection of several Fe/S proteins, which prevent
the release of iron ions and thereby avert further
damage. |
dc.language |
eng |
dc.publisher |
The American Society for Biochemistry and Molecular Biology |
dc.relation |
Reproducció del document publicat a https://doi.org/10.1074/jbc.M206525200 |
dc.relation |
The Journal of Biological Chemistry, 2002, vol. 277, núm 46, p. 44531-44538 |
dc.rights |
(c) The American Society for Biochemistry and Molecular Biology, 2002 |
dc.rights |
info:eu-repo/semantics/openAccess |
dc.title |
Mitochondrial Hsp60, resistance to oxidative stress, and the labile iron pool are closely connected in Saccharomyces cerevisiae |
dc.type |
article |
dc.type |
publishedVersion |