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Title:
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Protein Kinase KIS Localizes to RNA Granules and Enhances Local Translation
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Author:
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Cambray Carner, Serafí; Pedraza González, Neus; Rafel i Borrell, Marta; Garí Marsol, Eloi; Aldea, Martí; Gallego, Carme
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Notes:
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The regulation of mRNA transport is a fundamental process for cytoplasmic sorting of transcripts and
spatially controlled translational derepression once properly localized. There is growing evidence that translation
is locally modulated as a result of specific synaptic inputs. However, the underlying molecular mechanisms
that regulate this translational process are just emerging. We show that KIS, a serine/threonine kinase
functionally related to microtubule dynamics and axon development, interacts with three proteins found in
RNA granules: KIF3A, NonO, and eEF1A. KIS localizes to RNA granules and colocalizes with the KIF3A
kinesin and the β-actin mRNA in cultured cortical neurons. In addition, KIS is found associated with KIF3A
and 10 RNP-transported mRNAs in brain extracts. The results of knockdown experiments indicate that KIS
is required for normal neurite outgrowth. More important, the kinase activity of KIS stimulates 3' untranslated
region-dependent local translation in neuritic projections. We propose that KIS is a component of the
molecular device that modulates translation in RNA-transporting granules as a result of local signals. |
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Rights:
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(c) American Society for Microbiology, 2009
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Document type:
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article
publishedVersion |
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Published by:
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American Society for Microbiology
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