dc.contributor.author |
Machín, Félix |
dc.contributor.author |
Torres Rosell, Jordi |
dc.contributor.author |
Jarmuz, Adam |
dc.contributor.author |
Aragón, Luis |
dc.date |
2015-04-23T12:28:10Z |
dc.date |
2015-04-23T12:28:10Z |
dc.date |
2005 |
dc.identifier |
0021-9525 |
dc.identifier |
http://hdl.handle.net/10459.1/48158 |
dc.identifier |
https://doi.org/10.1083/jcb.200408087 |
dc.identifier.uri |
http://hdl.handle.net/10459.1/48158 |
dc.description |
Mitotic cell division involves the equal segregation
of all chromosomes during anaphase. The
presence of ribosomal DNA (rDNA) repeats on
the right arm of chromosome XII makes it the longest in
the budding yeast genome. Previously, we identified a
stage during yeast anaphase when rDNA is stretched
across the mother and daughter cells. Here, we show that
resolution of sister rDNAs is achieved by unzipping of the
locus from its centromere-proximal to centromere-distal
regions. We then demonstrate that during this stretched
stage sister rDNA arrays are neither compacted nor segregated
despite being largely resolved from each other.
Surprisingly, we find that rDNA segregation after this
period no longer requires spindles but instead involves
Cdc14-dependent rDNA axial compaction. These results
demonstrate that chromosome resolution is not simply a
consequence of compacting chromosome arms and that
overall rDNA compaction is necessary to mediate the
segregation of the long arm of chromosome XII. |
dc.language |
eng |
dc.publisher |
The Rockefeller University Press |
dc.relation |
Reproducció del document publicat a https://doi.org/10.1083/jcb.200408087 |
dc.relation |
Journal of Cell Biology, 2005, vol. 168, núm. 2, p. 209-219 |
dc.rights |
by-nc-sa (c) Machín et al., 2005 |
dc.rights |
http://creativecommons.org/licenses/by-nc-sa/3.0/ |
dc.rights |
info:eu-repo/semantics/openAccess |
dc.title |
Spindle-independent condensation-mediated segregation of yeast ribosomal DNA in late anaphase |
dc.type |
article |
dc.type |
publishedVersion |