To access the full text documents, please follow this link: http://hdl.handle.net/10459.1/48153

Transcription of ribosomal genes can cause nondisjunction
Machín, Felix; Torres Rosell, Jordi; Piccoli, Giacomo De; Carballo, Jesús A.; Cha, Rita S.; Jarmuz, Adam; Aragón, Luis
Mitotic disjunction of the repetitive ribosomal DNA (rDNA) involves specialized segregation mechanisms dependent on the conserved phosphatase Cdc14. The reason behind this requirement is unknown. We show that rDNA segregation requires Cdc14 partly because of its physical length but most importantly because a fraction of ribosomal RNA (rRNA) genes are transcribed at very high rates. We show that cells cannot segregate rDNA without Cdc14 unless they undergo genetic rearrangements that reduce rDNA copy number. We then demonstrate that cells with normal length rDNA arrays can segregate rDNA in the absence of Cdc14 as long as rRNA genes are not transcribed. In addition, our study uncovers an unexpected role for the replication barrier protein Fob1 in rDNA segregation that is independent of Cdc14. These fi ndings demonstrate that highly transcribed loci can cause chromosome nondisjunction.
2006
http://creativecommons.org/licenses/by-nc-sa/3.0/
by-nc-sa (c) Machín et al., 2006
article
The Rockefeller University Press
         

Full text files in this document

Files Size Format View
009301.pdf 4.088 MB application/pdf View/Open

Show full item record

Related documents

Other documents of the same author

 

Coordination

 

Supporters