Título:
|
The incC sequence is required for R27 plasmid stability
|
Autor/a:
|
Tassinari, Eleonora; Aznar Vicente, Sonia; Urcola, Imanol; Prieto, Alejandro; Hüttener Queiroz, Mário; Juárez Giménez, Antonio
|
Abstract:
|
IncHI plasmids account for multiple antimicrobial resistance in Salmonella and other enterobacterial genera. These plasmids are generally very stable in their bacterial hosts. R27 is the archetype of IncHI1 plasmids. A high percentage of the R27-encoded open reading frames (ORFs) (66.7%) do not show similarity to any known ORFs. We performed a deletion analysis of all non-essential R27 DNA sequences to search for hitherto non-identified plasmid functions that might be required for plasmid stability. We report the identification of a short DNA sequence (incC) that is essential for R27 stability. That region contains several repeats (incC repeats), belongs to one of the three-plasmid replicons (R27 FIA-like) and is targeted by the R27 E protein. Deletion of the incC sequence drastically reduces R27 stability both in Escherichia coli and in Salmonella, the effect being more pronounced in this latter species. Interfering with incC-E protein interaction must lead to a reduced IncHI1 plasmid stability, and may represent a new approach to combat antimicrobial resistance. |
Materia(s):
|
-Seqüència d'aminoàcids -Plasmidis -Amino acid sequence -Plasmids |
Derechos:
|
cc-by (c) Tassinari, Eleonora et al., 2016
http://creativecommons.org/licenses/by/3.0/es |
Tipo de documento:
|
Artículo Artículo - Versión publicada |
Editor:
|
Frontiers Media
|
Compartir:
|
|