Title:
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Minimal genetic change in Vibrio cholerae in Mozambique over
time: Multilocus variable number tandem repeat analysis and
whole genome sequencing
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Author:
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Garrine, Marcelino; Mandomando, Inácio; Vubil, Delfino; Nhampossa, Tacilta; Acácio, Sozinho; Li, Shan; Paulson, Joseph N.; Almeida, Mathieu; Domman, Daryl; Thomson, Nicholas R.; Alonso, Pedro; Stine, Oscar C.
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Abstract:
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Although cholera is a major public health concern in Mozambique,
its transmission patterns remain unknown. We surveyed the
genetic relatedness of 75 Vibrio cholerae isolates from patients
at Manhica District Hospital between 2002-2012 and 3 isolates
from river using multilocus variable-number tandem-repeat
analysis (MLVA) and whole genome sequencing (WGS). MLVA revealed
22 genotypes in two clonal complexes and four unrelated
genotypes. WGS revealed i) the presence of recombination, ii) 67
isolates descended monophyletically from a single source
connected to Wave 3 of the Seventh Pandemic, and iii) four
clinical isolates lacking the cholera toxin gene. This Wave 3
strain persisted for at least eight years in either an
environmental reservoir or circulating within the human
population. Our data raises important questions related to where
these isolates persist and how identical isolates can be
collected years apart despite our understanding of high change
rate of MLVA loci and the V. cholerae molecular clock. |
Subject(s):
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-Còlera -Moçambic -Cholera -Mozambique |
Rights:
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cc by (c) Garrine et al., 2017
http://creativecommons.org/licenses/by/4.0/ |
Document type:
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Article Article - Published version |
Published by:
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Public Library of Science
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