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Title:
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Electromechanical conditioning of adult progenitor cells improves recovery of cardiac function after myocardial infarction
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Author:
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Llucia Valldeperes, A.; Soler Botija, Carolina; Gálvez Montón, Carolina; Roura, Santiago; Prat Vidal, Cristina; Perea Gil, Isaac; Sánchez Terrones, Benjamin; Bragós Bardia, Ramon; Vunjak-Novakovic, Gordana; Bayés Genis, Antoni
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Other authors:
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Universitat Politècnica de Catalunya. Departament d'Enginyeria Electrònica; Universitat Politècnica de Catalunya. IEB - Instrumentació Electrònica i Biomèdica |
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Abstract:
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Cardiac cells are subjected to mechanical and electrical forces, which regulate gene expression and cellular function. Therefore, in vitro electromechanical stimuli could benefit further integration of
therapeutic cells into the myocardium. Our goals were (a) to study the viability of a tissue engineered construct with cardiac adipose tissue-derived progenitor cells (cardiac ATDPCs) and (b) to examine the effect of electromechanically stimulated cardiac ATDPCs within a myocardial infarction (MI) model inmice for the first time. Cardiac ATDPCs were electromechanically stimulated at 2-millisecond pulses of 50 mV/cm at 1 Hz and 10% stretching during 7 days. The cells were harvested,
labeled, embedded in a fibrin hydrogel, and implanted over the infarcted area of the murine heart. A total of 39 animals were randomly distributed and sacrificed at 21 days: groups of grafts without cells and with stimulated or nonstimulated cells. Echocardiography and gene and protein analyses were also carried out. Physiologically stimulated ATDPCs showed increased expression of cardiac transcription factors, structural genes, and calcium handling genes. At 21 days after implantation, cardiac function (measured as left ventricle ejection fraction between presacrifice and post-MI) increased up to 12% in stimulated grafts relative to nontreated animals. Vascularization and integration with the host
blood supply of grafts with stimulated cells resulted in increased vessel density in the infarct border region. Trained cells within the implanted fibrin patch expressed main cardiac markers and migrated into the underlying ischemic myocardium. To conclude, synchronous electromechanical cell conditioning before delivery may be a preferred alternative when considering strategies for heart repair after myocardial infarction. |
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Abstract:
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Peer Reviewed |
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Subject(s):
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-Àrees temàtiques de la UPC::Ciències de la salut
-Stem cells
-Tissue engineering
-Cardiac regeneration
-Electromechanical conditioning
-Biomedical Engineering
-Myocardial infarction
-Cèl·lules mare |
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Rights:
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Document type:
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Article - Submitted version
Article |
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