Knowledge and accurate prediction of physiochemical properties of liquid mixtures is of great importance for understanding intermolecular interactions—interactions which determine the macroscopic performance of liquid fuels used in modern high performance engines. Accurate prediction of viscosity and surface tension for binary mixtures of components with marked property differences remains a challenging task due to the nonlinearity and sensitivity of the detailed molecular interactions of the blend components. This challenge is compounded by the non-Newtonian fluid effects that begin to develop as seed-particles are added to the flow. For this work, detailed fluid measurements of viscosity, density, and surface tension were carried out using rigorously prepared binary mixtures of dipropylene glycol and water at nominal lab temperatures. In addition, a selection of 5 glycol/water blends were tested in the Chalmers steady spray rig under turbulent flow conditions. Here, microscale fluorescent particle velocimetry was applied to measure the internal velocity profile in a plain-orifice nozzle. These flows generated by the spray rig are central to several ongoing projects at Combustion, and highly sensitive to fluid properties. These data and the subsequent property model comparisons of this work directly support the primary breakup studies of the spray group at Combustion and studies of pulsating and constrained flows at Fluid Dynamics.