Autor/a:
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Annamalay, Alicia A.; Lanaspa, Miguel; Khoo, Siew-Kim; Madrid, Lola; Acácio, Sozinho; Zhang, Guicheng; Laing, Ingrid; Gern, James; Goldblatt, Jack; Bizzintino, Joelene; Lehmann, Deborah; Le Souef, Peter N.; Bassat Orellana, Quique
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Abstract:
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OBJECTIVES: To describe the prevalence of human rhinovirus (RV)
species in children hospitalized with pneumonia in Manhica,
Mozambique; and the associations between RV species and
demographic, clinical, and laboratory features. METHODS:
Nasopharyngeal aspirates were collected from children 0-10 years
of age (n=277) presenting to Manhica District Hospital with
clinical pneumonia. Blood samples were collected for HIV and
malaria testing, blood culture and full blood counts, and a
chest X-ray was performed. A panel of common respiratory viruses
was investigated using two independent multiplex RT-PCR assays
with primers specific for each virus and viral type. RV species
and genotypes were identified by semi-nested PCR assays,
sequencing and phylogenetic tree analyses. RESULTS: At least one
respiratory virus was identified in 206 (74.4%) children
hospitalized with clinical pneumonia. RV was the most common
virus identified in both HIV-infected (17/38, 44.7%) and
HIV-uninfected (74/237, 31.2%; p=0.100) children. RV-A was the
most common RV species identified (47/275, 17.0%), followed by
RV-C (35/275, 12.6%) and RV-B (8/275, 2.9%). Clinical
presentation of the different RV species was similar and
overlapping, with no particular species being associated with
specific clinical features. CONCLUSIONS: RV-A and RV-C were the
most common respiratory viruses identified in children
hospitalised with clinical pneumonia in Manhica. Clinical
presentation of RV-A and RV-C was similar and overlapping. This
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