The impact of hedonic evaluation on Spanish consumers’ preferences towards beef attributes including its enrichment with polyunsaturated fatty acids (n-3: omega-3 and CLA: conjugated linoleic acid) was analysed using choice experiments (CE). 642 consumers from three Spanish cities were divided into two groups and half of them received information about n-3 and CLA fatty acids. Consumers evaluated the relative importance of beef attributes and their levels by conducting a CE, subsequently tasted four different beef samples and concluded by repeating the CE. Results showed higher consumers’ overall acceptability scores for beef enriched with n-3 and CLA than conventional beef, and the information offered to consumers had no significance impact on their acceptability scores. However, the hedonic evaluation had a significant impact on consumer beef preferences, in particular, for the animal diet attribute. The utility for beef enriched with n-3 fatty acids increased significantly after tasting, especially for the non-informed consumers.