Title:
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Influence of fabrication parameters on cellular microarrays for stem cell studies
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Author:
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Rodríguez-Seguí, S.A.; Pla-Roca, M.; Engel López, Elisabeth; Planell Estany, Josep Anton; Martínez, E.; Samitier Martí, Josep
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Other authors:
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Universitat Politècnica de Catalunya. Departament de Ciència dels Materials i Enginyeria Metal·lúrgica; Institut de Bioenginyeria de Catalunya; Universitat Politècnica de Catalunya. BBT - Biomaterials, Biomecànica i Enginyeria de Teixits |
Abstract:
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Lately there has been an increasing interest in
the development of tools that enable the high throughput
analysis of combinations of surface-immobilized signaling
factors and which examine their effect on stem cell biology
and differentiation. These surface-immobilized factors
function as artificial microenvironments that can be
ordered in a microarray format. These microarrays could be
useful for applications such as the study of stem cell
biology to get a deeper understanding of their differentia-
tion process. Here, the evaluation of several key process
parameters affecting the cellular microarray fabrication is
reported in terms of its effects on the mesenchymal stem
cell culture time on these microarrays. Substrate and pro-
tein solution requirements, passivation strategies and cell
culture conditions are investigated. The results described in
this article serve as a basis for the future development of
cellular microarrays aiming to provide a deeper under-
standing of the stem cell differentiation process. |
Abstract:
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Peer Reviewed |
Subject(s):
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-Àrees temàtiques de la UPC::Enginyeria dels materials -DNA microarrays -Microxips d'ADN -Cèl·lules |
Rights:
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Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/ |
Document type:
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Article - Published version Article |
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