Título:
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Preparation of functional DNA microarrays through laser-induced forward transfer
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Autor/a:
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Serra Coromina, Pere; Colina Brito, Mónica Alejandra; Fernández Pradas, Juan Marcos; Sevilla, Lidia; Morenza, J. Luis
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Otros autores:
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Universitat Politècnica de Catalunya. Departament d'Enginyeria Electrònica |
Abstract:
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A functional DNAmicroarray was prepared through the laser-induced forward transfer (LIFT) technique. In a first experiment, droplets of a buffer solution were spotted onto a substrate at different laser pulse energies. This allowed one to determine that uniform spots with a diameter as small as 40μm could be obtained. In a second experiment, a microarray containing two different human cDNAs and a negative control was spotted through LIFT and submitted to a hybridization assay. The obtained results demonstrated the full functionality of the microarray, which allowed us to prove the viability of LIFT for the production of DNAmicroarrays. |
Materia(s):
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-Àrees temàtiques de la UPC::Física -Àrees temàtiques de la UPC::Ciències de la visió::Òptica física -Lasers -ADN -Làsers -- Aplicacions científiques |
Derechos:
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Tipo de documento:
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Artículo - Versión publicada Artículo |
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