Títol:
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Automatic system for electroporation of adherent cells growing in standard multi-well plates
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Autor/a:
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García Sánchez, Tomás; Guitart, Maria; Rosell Ferrer, Francisco Javier; Gomez Foix, Anna Maria; Bragós Bardia, Ramon
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Altres autors:
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Universitat Politècnica de Catalunya. Departament d'Enginyeria Electrònica; Universitat Politècnica de Catalunya. IEB - Instrumentació Electrònica i Biomèdica |
Abstract:
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In this study an automatic system is presented to perform electroporation, also known as electropermeabilization,
on adherent cells. It is an intention of this system to apply electric field pulses directly to cells growing in standard multiwell plates as a step forward to include this technique in standard laboratory protocols. An interdigitated microelectrode assembly constructed with Printed Circuit Board (PCB) is placed closely above the cell monolayer, and in order to avoid direct contact with cells, small micro-separators were included
in the structure. Additionally, distribution of current density was modified by filling the gap between adjacent electrodes with a non conductive material as predicted by electric field simulations. This modification helps to concentrate the electric field intensity in the region where cells are present. The device was tested using C2C12 cell line growing adhered in 24 multiwell plates and fluorescent labeled dextran FD20S as the molecule to be delivered. Successful transfection was observed with minimal invasiveness of the operation reducing the stress caused to cells. |
Matèries:
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-Àrees temàtiques de la UPC::Enginyeria electrònica -Àrees temàtiques de la UPC::Ciències de la salut -Electroporation. -Electroporació |
Drets:
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Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/ |
Tipus de document:
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Article - Versió publicada Objecte de conferència |
Publicat per:
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IEEE
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