The aim of our work was to determine the zeta potentials of an EMPA-type standard cotton fabric(dyed, finished and softened) washed in the presence of surfactants and a dye transfer inhibitor(DTI) copolymer. The DTI copolymer used was of the VI/PVP type. The fabric was washed with ecological surfactants: sodium dodecylbenzenesulfonate (LAS), anionic, mixed with alkylpolyglucoside (APG), non-ionic. The cotton fabric was dyed with three different direct dyes(with different molecular weights), finished with a DMDHEU resin, and softened with an ammonium silicone microemulsion compound. The variation of the zeta potential of the cotton fabrics was obtained under the influence of the total concentration and proportions of the mixture of the anionic and non-ionic surfactants used together and separately. The DTI polymer was kept at a constant concentration in the mixture with the surfactants. We obtained the variation of the zeta potential of the undyed and finished cotton fabrics as a function of the DTI.