Title:
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Generation of biological association networks: A novel strategy to detect new targets in cancer therapy
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Author:
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Selga i Coma, Elisabet; Almagro García, Ma. Cristina de; Oleaga, Carlota; Mencial, Núria; Ramírez, Sara; Ruiz, F. Xavier; Farrés i Vicén, Jaume; Parés i Casasampera, Xavier; Thibaut, Rémi; Porte Visa, Cinta; Noé Mata, Verónica; Ciudad i Gómez, Carlos Julián
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Other authors:
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Universitat de Barcelona |
Abstract:
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Podeu consultar el llibre complet a: http://www.trnres.com/ebookcontents.php?id=149 |
Abstract:
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The aim of this work was to design a novel strategy to detect new targets for anticancer treatments. The rationale was to build Biological Association Networks from differentially expressed genes in drug-resistant cells to identify important nodes within the Networks. These nodes may represent putative targets to attack in cancer therapy, as a way to destabilize the gene network developed by the resistant cells to escape from the drug pressure. As a model we used cells resistant to methotrexate (MTX), an inhibitor of DHFR. Selected node-genes were analyzed at the transcriptional level and from a genotypic point of view. In colon cancer cells, DHFR, the AKR1 family, PKC¿, S100A4, DKK1, and CAV1 were overexpressed while E-cadherin was lost. In breast cancer cells, the UGT1A family was overexpressed, whereas EEF1A1 was overexpressed in pancreatic cells. Interference RNAs directed against these targets sensitized cells towards MTX. |
Subject(s):
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-Càncer -Quimioteràpia -Farmacogenètica -Cancer -Chemotherapy -Pharmacogenetics |
Rights:
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(c) Transworld Research Network, 2011
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Document type:
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Book Part |
Published by:
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Transworld Research Network
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