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Title:
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Sit4 is required for proper modulation of the biological functions mediated by Pkc1 and the cell integrity pathway in Saccharomyces cerevisiae
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Author:
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Torre Ruiz, M. A. de la; Torres Rosell, Jordi; Ariño Carmona, Joaquín; Herrero Perpiñán, Enrique
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Notes:
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Maintenance of cellular integrity in Saccharomyces
cerevisiae is carried out by the activation of the protein
kinase C-mediated mitogen-activated protein kinase
(PKC1-MAPK) pathway. Here we report that correct
down-regulation of both basal and induced activity of
the PKC1-MAPK pathway requires the SIT4 function.
Sit4 is a protein phosphatase also required for a proper
cell cycle progression. We present evidence demonstrat-
ing that the G1 to S delay in the cell cycle, which occurs
as a consequence of the absence of Sit4, is mediated by
up-regulation of Pkc1 activity. Sit4 operates down-
stream of the plasma membrane sensors Mid2, Wsc1, and
Wsc2 and upstream of Pkc1. Sit4 affects all known bio-
logical functions involving Pkc1, namely Mpk1 activity
and cell wall integrity, actin cytoskeleton organization,
and ribosomal gene transcription. |
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Rights:
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(c) The American Society for Biochemistry and Molecular Biology, 2002
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Document type:
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article
publishedVersion |
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Published by:
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The American Society for Biochemistry and Molecular Biology
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