|
Title:
|
Biphasic modulation of fatty acid synthase by hydrogen peroxide in Saccharomyces cerevisiae
|
|
Author:
|
Matias, Ana C.; Marinho, H. Susana; Cyrne, Luísa; Herrero Perpiñán, Enrique; Antunes, Fernando
|
|
Notes:
|
Taking into account published contradictory results concerning the regulation of fatty acid synthase (Fas)
by H2O2, we carried out a systematic study where two methods of H2O2 delivery (steady-state and bolus
addition) and the effect of a wide range of H2O2 concentrations were investigated. A decrease in Fas activity
was observed for cells exposed to 100 and 150 lMH2O2 in a steady-state, while a bolus addition of the
same H2O2 concentrations did not alter Fas activity. Similar results were observed for the mRNA levels of
FAS1, the gene that encodes Fas subunit b. However, the exposure to a steady-state 50 lMH2O2 dose lead
to an increase in FAS1 mRNA levels, showing a biphasic modulation of Fas by H2O2. The results obtained
emphasize that cellular effects of H2O2 can vary over a narrow range of concentrations. Therefore, a tight
control of H2O2 exposure, which can be achieved by exposing H2O2 in a steady-state, is important for cellular
studies of H2O2-dependent redox regulation. |
|
Subject(s):
|
-H2O2
-Adaptation
-Fatty acid synthase |
|
Rights:
|
(c) Elsevier, 2011
info:eu-repo/semantics/restrictedAccess |
|
Document type:
|
article
publishedVersion |
|
Published by:
|
Elsevier
|
|
Share:
|
|
