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Title:
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Transcription of ribosomal genes can cause nondisjunction
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Author:
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Machín, Félix; Torres Rosell, Jordi; Piccoli, Giacomo De; Carballo, Jesús A.; Cha, Rita S.; Jarmuz, Adam; Aragón, Luis
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Notes:
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Mitotic disjunction of the repetitive ribosomal
DNA (rDNA) involves specialized segregation
mechanisms dependent on the conserved phosphatase
Cdc14. The reason behind this requirement is
unknown. We show that rDNA segregation requires
Cdc14 partly because of its physical length but most
importantly because a fraction of ribosomal RNA (rRNA)
genes are transcribed at very high rates. We show that cells
cannot segregate rDNA without Cdc14 unless they undergo
genetic rearrangements that reduce rDNA copy number.
We then demonstrate that cells with normal length rDNA
arrays can segregate rDNA in the absence of Cdc14 as
long as rRNA genes are not transcribed. In addition, our
study uncovers an unexpected role for the replication barrier
protein Fob1 in rDNA segregation that is independent
of Cdc14. These fi ndings demonstrate that highly transcribed
loci can cause chromosome nondisjunction. |
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Rights:
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by-nc-sa (c) Machín et al., 2006
http://creativecommons.org/licenses/by-nc-sa/3.0/
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Document type:
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article
publishedVersion |
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Published by:
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The Rockefeller University Press
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